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Gradient sperm selection for reproductive techniques in cattle: Is Isolate a suitable replacement for Percoll?
Author(s) -
Sepúlveda B.,
Arias M. E.,
Aguila L.,
Zambrano F.,
Sánchez R.,
Felmer R.
Publication year - 2018
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/and.12921
Subject(s) - percoll , andrology , sperm , acrosome , biology , dna fragmentation , semen , cryopreservation , acrosome reaction , sperm motility , fertilisation , motility , reproductive technology , embryo , anatomy , microbiology and biotechnology , genetics , in vitro , medicine , apoptosis , programmed cell death
Summary In assisted reproductive techniques, it is essential to perform a sperm selection to obtain spermatozoa with high motility and membrane integrity for in vitro fertilisation ( IVF ) and high‐ DNA integrity for intracytoplasmic sperm injection ( ICSI ). In this study, we evaluated whether Isolate ® was a suitable substitute for Percoll ® for assisted reproductive techniques. Commercial cryopreserved bovine semen was used after selection in both gradients, and plasma and acrosome membrane integrity, reactive oxygen species ( ROS ) levels, DNA integrity and mitochondrial membrane potential (ΔΨm) were assessed by flow cytometry. Motility parameters were also evaluated by CASA system. A similar percentage of spermatozoa with intact plasma membrane, acrosome integrity and high ΔΨm was observed in both sperm selection methods, but only Percoll ® showed higher percentage of spermatozoa with intact plasma and acrosome membrane compared to the post‐thawing group. No differences were observed in the motility, ROS , DNA fragmentation and on the in vitro embryo production in all experimental groups. In conclusion, the selection of bovine spermatozoa with Isolate ® generates spermatozoa with similar quality parameters and embryonic development compared to Percoll ® providing a suitable alternative sperm selection method for assisted reproductive techniques in this species.