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Effects of testosterone enanthate and resistance training on myocardium in Wistar rats; clinical and anatomical pathology
Author(s) -
Karbasi S.,
Zaeemi M.,
Mohri M.,
Rashidlamir A.,
Moosavi Z.
Publication year - 2018
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/and.12908
Subject(s) - creatine kinase , lactate dehydrogenase , troponin i , endocrinology , medicine , testosterone (patch) , creatinine , muscle hypertrophy , placebo , histopathology , chemistry , pathology , enzyme , biochemistry , alternative medicine , myocardial infarction
Summary This study was performed to determine the effects of 8 weeks testosterone enanthate (TE) injection and resistance training (RT) on cardiac muscle in male Wistar rats. A total of 28 male adult Wistar rats were randomly divided into 4 groups; control + placebo, RT + placebo, TE and TE + RT. Testosterone enanthate (20 mg/kg BW, IM) and placebo (olive oil; 0.2 ml, IM) were injected twice a week for 2 months. The RT consisted of climbing (5 reps/3 sets) a ladder carrying a load suspended from the tail. The serum activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH) and creatine kinase MB (CK‐MB) and serum level of creatinine, urea and cardiac troponin I (CTnI) were evaluated. After sacrifice, samples from myocardial muscle were collected for histopathology evaluation. The serum concentration of CTnI and CK‐MB activity significantly increased in group RT compared with control ( p < .05). In group RT + TE, all biomarkers of muscle damage (CTnI, CK‐MB, AST, LDH) were significantly more than those in control ( p < .05). Also, mild myocardial hypertrophy was observed in RT and RT + TE groups. The higher level of all heart damage biomarkers in the RT + TE group rather than control may indicate the synergistic effects of medication and exercise.