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Freezability of water buffalo bull ( Bubalus bubalis ) spermatozoa is improved with the addition of curcumin (diferuoyl methane) in semen extender
Author(s) -
Shah S. A. H.,
Andrabi S. M. H.,
Qureshi I. Z.
Publication year - 2017
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/and.12713
Subject(s) - extender , curcumin , semen extender , semen , andrology , acrosome , chemistry , sperm , antioxidant , sperm motility , zoology , biology , biochemistry , medicine , organic chemistry , polyurethane
Summary Effects of curcumin as antioxidant in extender were evaluated on freezability of buffalo spermatozoa. Semen from each of the five bulls ( n = 3 replicates, six ejaculates/bull, a total of 30 ejaculates) was diluted in Tris ‐citric acid extender containing curcumin (0.5, 1.0, 1.5 or 2.0 mM) or control. At pre‐freezing and post‐thawing, total antioxidant contents (μM/L) and lipid peroxidation levels (μM/ml) were higher ( p < .05) and lower ( p < .05) respectively, with 1.5 and 2.0 mM compared to 0.5 and 1.0 mM curcumin and control. At post‐thawing, progressive motility (PM, %) and rapid velocity ( RV , %) were higher ( p < .05) with 1.5 mM compared to other doses of curcumin and control (except in case of RV , 1.5 was similar with 1.0 mM). Kinematics (average path velocity, μm/s; straight‐line velocity, μm/s; curved‐line velocity, μm/s; straightness, %; linearity, %), in vitro longevity (%, PM and RV) and DNA integrity (%) at post‐thawing were higher ( p < .05) with 1.5 mM compared to control. At post‐thawing, supravital plasma membrane integrity (%) and viable spermatozoa with intact acrosome (%) were higher with 1.5 compared to 2.0 mM curcumin and control. We concluded that freezability of water buffalo spermatozoa is improved with the addition of 1.5 mM curcumin in extender.