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Effects of glycerol, equilibration time and antioxidants on post‐thaw functional integrity of bovine spermatozoa directly obtained from epididymis
Author(s) -
Almeida F. C.,
Silva S. V.,
Souza H. M.,
Gomes W. A.,
Lima Filho J. A. C.,
Wicke A. A.,
Batista A. M.,
Guerra M. M. P.
Publication year - 2017
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/and.12623
Subject(s) - extender , sperm , acrosome , chemistry , glycerol , andrology , superoxide dismutase , yolk , tris , epididymis , sperm motility , semen , catalase , biochemistry , antioxidant , food science , medicine , organic chemistry , polyurethane
Summary This work aimed to evaluate the effect of stabilisation times, glycerol concentration, and the catalase and superoxide dismutase supplementation of diluent on parameters of frozen‐thawed spermatozoa from epididymis of Nelore bulls: Experiment 1: spermatozoa diluted in Tris‐egg yolk with glycerol (3%, 5% or 7%) and stabilisation times (0, 2 or 4 hr at 5°C); Experiment 2: Tris‐egg yolk only, Tris‐egg yolk with catalase ( CAT , 50 or 100 U ml −1 ) or superoxide dismutase ( SOD , 50 or 100 U ml −1 ). Frozen‐thawed spermatozoa were evaluated for kinetic parameters, plasma membrane and acrosome integrity, mitochondrial activity and IVF capacity. ALH and BCF were affected ( p < .05) by glycerol at 3% after 4‐hr equilibration time and 7% after 2‐hr equilibration time. Glycerol 3% had lower ( p < .05) iPM and iA c after 4 hr. Glycerol 5% had greater ( p < .05) hPMM after 4 hr and iA c after 2 hr than at 0 hr. SOD 100 U ml −1 had lower ( p < .05) linearity and wobble compared to control group. No was observed differences to fertilisation rate ( p < .05) among groups. In conclusion, glycerol 5% in Tris‐egg yolk extender for 4 hr is suitable for the preservation of sperm kinetics and membrane integrity. CAT (50 and 100 U ml −1 ) or SOD (50–100 U ml −1 ) had no beneficial effects on sperm kinetics, plasma and acrosomal membrane integrity, mitochondrial activity or the capacity for IVF of frozen‐thawed spermatozoa from epididymis of Nelore bulls.