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l ‐Cysteine improves antioxidant enzyme activity, post‐thaw quality and fertility of Nili‐Ravi buffalo ( Bubalus bubalis ) bull spermatozoa
Author(s) -
Iqbal S.,
Riaz A.,
Andrabi S. M. H.,
Shahzad Q.,
Durrani A. Z.,
Ahmad N.
Publication year - 2016
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/and.12520
Subject(s) - extender , andrology , antioxidant , chemistry , glutathione peroxidase , cysteine , acrosome , biology , zoology , semen , biochemistry , catalase , enzyme , medicine , organic chemistry , polyurethane
Summary The effects of l ‐cysteine in extender on antioxidant enzymes profile during cryopreservation, post‐thaw quality parameters and in vivo fertility of Nili‐Ravi buffalo bull spermatozoa were studied. Semen samples from 4 buffalo bulls were diluted in Tris–citric acid‐based extender having different concentrations of l ‐cysteine (0.0, 0.5, 1.0, 2.0 and 3.0 m m ) and frozen in 0.5‐ml French straws. The antioxidative enzymes [catalase, super oxide dismutase and total glutathione (peroxidase and reductase)] were significantly higher ( P < 0.05) at pre‐freezing and post‐thawing in extender containing 2.0 m m l ‐cysteine as compared to other groups. Post‐thaw total motility (%), progressive motility (%), rapid velocity (%), average path velocity (μm s −1 ), straight line velocity (μm s −1 ), curvilinear velocity (μm s −1 ), beat cross frequency (Hz), viable spermatozoa with intact plasmalemma (%), acrosome and DNA integrity (%) were higher with the addition of 2.0 m m l ‐cysteine as compared to other groups ( P < 0.05). The fertility rates (59 versus 43%) were higher ( P < 0.05) in buffaloes inseminated with doses containing 2.0 m m of l ‐cysteine than in the control. In conclusion, the addition of 2.0 m m l ‐cysteine in extender improved the antioxidant enzymes profile, post‐thaw quality and in vivo fertility of Nili‐Ravi buffalo bull spermatozoa.