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Cadmium inhibits motility, activities of plasma membrane Ca 2+ ‐ ATP ase and axonemal dynein‐ ATP ase of human spermatozoa
Author(s) -
Da Costa R.,
Botana D.,
Piñero S.,
Proverbio F.,
Marín R.
Publication year - 2016
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/and.12466
Subject(s) - motility , sperm motility , dynein , atpase , sperm , biology , dynein atpase , chemistry , microbiology and biotechnology , biochemistry , enzyme , microtubule , botany
Summary Cd 2+ has been associated with decreased sperm motility in individuals exposed to this element, such as smokers. Among other factors, this lowered motility could be the result of inhibition exerted by Cd 2+ on the activity of the sperm ATP ases associated with sperm motility. In this study, we evaluated the plasma membrane Ca 2 + ‐ ATP ase and the axonemal dynein‐ ATP ase activities as well as sperm motility, in the presence of different free Cd 2+ concentrations in the assay media. It was found that spermatozoa incubated for 5 h in a medium containing 25 n m free Cd 2+ showed a significant inhibition of progressive motility, reaching values even lower at higher Cd 2+ concentrations. In addition, it was found that the activity of the plasma membrane Ca 2+ ‐ ATP ase reached maximal inhibition at 50 n m free Cd 2+ , with a K 50% inhibition of 18.3 n m free Cd 2+ . The dynein‐ ATP ase activity was maximally inhibited by 25 n m free Cd 2+ in the assay medium, with a K 50% inhibition of 11.3 n m Cd 2+ . Our results indicate that the decreased activity of the sperm ATP ases might have a critical importance in the biochemical mechanisms underlying the decreased sperm motility of individuals exposed to Cd 2+ .