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Evaluation of immunological interaction between spermatozoa and fallopian tube epithelial cells
Author(s) -
Zandieh Z.,
Ashrafi M.,
Jameie B.,
Amanpour S.,
Mosaffa N.,
Salman Yazdi R.,
Pacey A.,
Aflatoonian R.
Publication year - 2015
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/and.12391
Subject(s) - capacitation , fallopian tube , microbiology and biotechnology , tlr2 , cytokine , andrology , sperm , innate immune system , biology , tlr3 , semen , immune system , immunology , chemistry , toll like receptor , medicine , anatomy
Summary Toll‐like receptors ( TLR ) are one of the major compartments of innate immune system. It was revealed that the TLR have relevance in ovulation, sperm capacitation and fertilisation. So, in this study, the expression of TLR , their adaptor molecules and cytokines in human fallopian tube cell line under the effect of human normal spermatozoa was evaluated. TLR mRNA and protein were evaluated in OE ‐E6/E7 cell line. Semen samples from 10 donors were collected and co‐incubated with OE ‐E6/E7 cell line and used as sperm group, and cell line without spermatozoa was used as control group. Afterwards, the level of TLR , their adaptor molecule and cytokine mRNA expression was compared using qPCR in sperm and control groups, and supernatant was used for ELISA . To determine whether elevated cytokine reaction to spermatozoa in OE ‐E6/E7 cell line is mediated via TLR , TLR 3 function‐blocking antibody was used. OE ‐E6/E7 cell line expressed TLR 1–6 genes and proteins. TLR expressions, especially TLR 3 and TLR 5, in OE ‐E6/E7 cell line under the effect of spermatozoa were significantly higher. Also, levels of adaptor molecules and cytokine production were increased in sperm group than in control group ( P < 0.05). So, it may be hypothesised that TLR are essential for spermatozoa and fallopian tube immunological interaction and for preparing safe environment for important events in fallopian tube.