Premium
Joint effect of glutathione S‐transferase genotypes and cigarette smoking on idiopathic male infertility
Author(s) -
Yarosh S. L.,
Kokhtenko E. V.,
Churnosov M. I.,
Solodilova M. A.,
Polonikov A. V.
Publication year - 2015
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/and.12367
Subject(s) - gstp1 , genotype , male infertility , infertility , medicine , glutathione s transferase , genetics , gene , gynecology , biology , glutathione , pregnancy , enzyme , biochemistry
Summary Inconsistent results of association studies investigated the role of glutathione S–transferase genes in idiopathic male infertility may be explained by ethnical differences in gene–gene and gene–environment interactions. In this study, we investigated a joint contribution of GSTM 1 , GSTT 1 and GSTP 1 gene polymorphisms and cigarette smoking to the risk of idiopathic infertility in Russian men. DNA samples from 203 infertile and 227 fertile men were genotyped by a multiplex polymerase chain reaction ( GSTM 1 and GSTT 1 deletions) and PCR ‐restriction fragment length polymorphism ( GSTP 1 I105V) methods. The GSTP 1 genotype 105 IV was associated with increased risk of male infertility ( OR = 1.50 95% CI 1.02–2.20 P = 0.04). Genotype combinations GSTP 1 105 II / GSTT 1 del (G1), GSTM 1 del/ GSTT 1 del (G2) and GSTM 1 + / GSTT 1 del (G3) were associated with decreased risk of male infertility ( P ≤ 0.003), whereas a genotype combination GSTP 1 105 IV / GSTT 1 + (G4) was associated with increased disease risk ( P = 0.001). The genotype combinations G3 and G4 showed a significant association with infertility in smokers; however, nonsmokers carriers did show the disease risk. In conclusion, GSTM 1 , GSTT 1 and GSTP 1 genes are collectively involved in the development of idiopathic male infertility and their phenotypic effects on the disease risk are potentiated by cigarette smoking.