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Magnetic activated cell sorting: an effective method for reduction of sperm DNA fragmentation in varicocele men prior to assisted reproductive techniques
Author(s) -
Degheidy T.,
Abdelfattah H.,
Seif A.,
Albuz F. K.,
Gazi S.,
Abbas S.
Publication year - 2015
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/and.12343
Subject(s) - dna fragmentation , varicocele , andrology , semen , sperm , sperm motility , fragmentation (computing) , semen analysis , biology , motility , apoptosis , medicine , infertility , genetics , programmed cell death , pregnancy , ecology
Summary Semen parameters of varicocele men have been usually suspected to exhibit higher levels of abnormalities including DNA fragmentation, reactive oxygen species ( ROS ) and apoptotic markers. Negative correlation between increased level of DNA fragmentation and assisted reproductive techniques ( ART ) outcome has been studied by several authors. In the current study, we aim to evaluate the possible value of magnetic activated cell sorting ( MAC s) technology in reduction of DNA fragmentation in infertile varicocele patients prior to ART . Semen samples, collected from 36 varicocele patients, were prepared by density gradient centrifugation ( DGC ). Every sample was subsequently divided into two aliquots. One aliquot was kept untouched as pre‐ MAC s control while the other aliquot was subjected to MAC s technique, for depletion of apoptotic spermatozoa, and serves as post‐ MAC s test. Sperm count, motility and DNA fragmentations were evaluated for both control and test samples. Post‐ MAC s samples showed no deleterious reduction in total sperm motility (80.64 ± 6.97%) compared with control samples (80.97 ± 7.74%) while sperm DNA fragmentations were significantly reduced in post‐ MAC s samples (9.61 ± 5.62%) compared with pre‐ MAC s controls (12.43 ± 6.29%) ( P < 0.05). It can be concluded that MAC s technique is a simple, noninvasive, technique that can efficiently reduce DNA fragmentation in infertile varicocele patients prior to ART .