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A simple sperm nuclear vacuole assay with propidium iodide
Author(s) -
Zhu W.J.,
Li J.
Publication year - 2015
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/and.12328
Subject(s) - varicocele , propidium iodide , andrology , sperm , biology , intracytoplasmic sperm injection , staining , microbiology and biotechnology , medicine , genetics , infertility , in vitro fertilisation , embryo , pregnancy , programmed cell death , apoptosis
Summary Our aim was to develop a new simple sperm nuclear vacuole assay ( SNVA ) with propidium iodide ( PI ) to determine the status of nuclear vacuole ( NV ) of individual spermatozoa. After PI staining, sperm nuclei were classified into the 14 categories according to both nuclear morphology and the status of NV . The incidence was 57.8% (range 28–84%) in fertile controls ( n = 40), and 85.1% (range 67–99%) in men with varicocele ( n = 40). In the fertile group, normal nuclear‐shaped spermatozoa without NV or with one small NV located in the ante‐nuclear region were significantly more in comparison with the varicocele group. In the varicocele group, abnormal nuclear‐shaped spermatozoa with one large NV and with multiple NV s located in the ante‐nuclear region were most frequent findings. Besides, spermatozoa with NV s in both ante‐ and post‐nuclear regions in the varicocele group were significantly more than those in the fertile group. In both fertile and varicocele groups, normal or abnormal nuclear‐shaped spermatozoa with one or more vacuoles only located in the post‐nuclear region occurred sparingly. The SNVA provides a useful additional approach to identify the status of NV in human spermatozoa for diagnostic purposes. A good sperm sample would have more spermatozoa without NV or with one small NV located in the ante‐nuclear region.