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Xenotransplantation assessment: morphometric study of human spermatogonial stem cells in recipient mouse testes
Author(s) -
Mirzapour T.,
Movahedin M.,
Koruji M.,
Nowroozi M. R.
Publication year - 2015
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/and.12310
Subject(s) - xenotransplantation , andrology , spermatogenesis , biology , transplantation , stem cell , sertoli cell , homing (biology) , immunology , microbiology and biotechnology , medicine , endocrinology , ecology
Summary The purpose of this study was (i) To establish in vitro propagation of human spermatogonial stem cells ( hSSC s) from small testicular biopsies to obtain a high number of cells; (ii) to evaluate the presence of functional hSSC s in culture system by RT ‐ PCR using DAZL , α6‐Integrin, β1‐Integrin genes; and (iii) to evaluate the effects of cell concentration on successful xenotransplantation of hSSC s in mice testis. Donor hSSC s were obtained from men with maturation arrest of spermatogenesis duration 1 year ago. These cells were propagated in DMEM containing 1 ng ml −1 bFGF (basic fibroblast grow factor) and 1500 U ml LIF (leucaemia inhibitory factor) for 5 weeks. Different concentrations of hSSC s transplanted into seminiferous tubules of busulfan‐treated immunodeficient mice and analysed up to 8 weeks after transplantation. The results showed that expression of DAZL and α6‐Integrin mRNA was increased as well as the colony formation of SSCs in vtro culture during 5 weeks. Proliferation occurred about 4 weeks after transplantation, but meiotic differentiation was not observed in recipient testis after 8 weeks. The difference in donor cells concentration had effect on homing spermatogenesis in recipient testis. Homologous transplantation of proliferated SSC s to seminiferous tubules of that patient individually may allow successful differentiation of transplanted cells.