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Glutathione S‐transferase Mu‐1 gene polymorphism in Egyptian patients with idiopathic male infertility
Author(s) -
Roshdy O. H.,
Hussein T. M.,
Zakaria N. H.,
Sabry A. A.
Publication year - 2015
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/and.12306
Subject(s) - genotype , male infertility , glutathione s transferase , sperm , biology , medicine , infertility , restriction fragment length polymorphism , polymorphism (computer science) , andrology , gene polymorphism , glutathione , endocrinology , genetics , gene , enzyme , pregnancy , biochemistry
Summary The aim of this study was to examine whether an association exists between glutathione S‐transferase Mu‐1 ( GSTM 1) gene polymorphism and idiopathic male infertility. Sixty men with primary idiopathic infertility and 60 fertile men, serving as controls, were recruited for the study. The polymorphism was analysed using polymerase chain reaction–restriction fragment length polymorphism ( PCR ‐ RFLP ) technique. The frequency of GSTM 1 null genotype was observed to be higher in infertile men 40% in comparison with 33.3% in the fertile men, but this difference was not statistically significant. There was statistically significant difference between cases and controls as regards GSTM 1 genotype distribution ( MC P = 0.006*) in GSTM 1‐positive men. Patients with the GSTM 1 null genotype had significantly lower sperm concentrations and total sperm count when compared with patients with GSTM 1‐positive genotype. In the control group, men with GSTM 1 null genotype had significantly lower sperm concentrations but not total sperm count when compared with men with GSTM 1‐positive genotype. The results of this study suggest a possible negative effect of GSTM 1 null genotype on the spermatogenic potential of the testis.