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Effect of Camellia sinensis supplementation and increasing holding time on quality of cryopreserved boar semen
Author(s) -
Gale I.,
Gil L.,
Malo C.,
González N.,
Martínez F.
Publication year - 2015
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/and.12293
Subject(s) - extender , cryopreservation , camellia sinensis , andrology , semen , acrosome , sperm , semen cryopreservation , biology , lipid peroxidation , sperm motility , capacitation , food science , chemistry , zoology , antioxidant , botany , biochemistry , medicine , embryo , organic chemistry , polyurethane , microbiology and biotechnology
Summary Cryopreservation of boar semen is still considered suboptimal due to the low fertility when compared with fresh semen. This study was performed to evaluate the effects of green tea ( Camellia sinensis ) supplementation of the freezing extender at different concentration (0, 2.5%, 5%, 10%) and also to determine the influence of increasing holding time from 2 to 24 h at 15 °C. Seventeen ejaculates from nine boars were used to make pools of three of them and then cryopreserved. Sperm motility, viability, acrosome integrity, membrane functionality ( HOST ) and capacitation status were determined before freezing and at 0, 30, 60, 90 and 120 min after thawing. Lipid peroxidation was evaluated just after thawing. The main findings emerging from this study were the following: (i) no improvement in quality of thawed spermatozoa with addition of tea to the freezing extender, (ii) no improvement in quality of thawed spermatozoa with prolonged holding time, (iii) lower peroxidation rate in presence of tea 5% and (iv) a decrease in the number of uncapacited viable spermatozoa with any tea supplementation. We conclude that amplification of holding time in semen cryopreservation process does not vary results, facilitating freezing protocol. Tea supplementation reduces lipoxidation but did not improve quality parameters.