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Nuclear degraded sperm subpopulation is affected by poor chromatin compaction and nuclease activity
Author(s) -
RibasMaynou J.,
GarcíaPeiró A,
MartínezHeredia J.,
FernándezEncinas A.,
Abad C.,
Amengual M. J.,
Navarro J.,
Benet J.
Publication year - 2015
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/and.12258
Subject(s) - sperm , protamine , chromatin , nuclease , semen , andrology , nuclear dna , incubation , biology , cell nucleus , dna , chemistry , microbiology and biotechnology , biochemistry , nucleus , medicine , mitochondrial dna , heparin , gene
Summary There is an interest in the nuclear degraded sperm subpopulation because, although it is present in a low percentage in all semen samples, patient groups such as varicocele and rearranged genome carriers show high levels of these degraded spermatozoa. This study is designed with two objectives in mind: first, incubations of H 2 O 2 and nuclease on DTT ‐treated and untreated samples to show the aetiology of this subpopulation and second, assessment of the correlation between the protamine ratio and nuclear degraded spermatozoa. A very high increase in the nuclear degraded subpopulation has been found with nuclease incubation, and it is even higher when it has been merged with nuclear decompaction using DTT . Alternatively, incubation with H 2 O 2 with and without DTT did not show such a significant increase in nuclear degraded spermatozoa. The protamine ratio correlated with this subpopulation, showing, in patients, that poor nuclear compaction would turn the sperm susceptible to degradation. Then, the assessment of nuclear degraded spermatozoa might not be only a measure of DNA degradation but also an indicator of chromatin compaction in the spermatozoa. Different patient groups would fit this model for sperm nuclear degradation, such as varicocele patients, who show a high percentage of immature spermatozoa and nuclear degraded spermatozoa, and reorganised genome carriers, where reorganisation might also cause poor chromatin compaction on the sperm nucleus.