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Association of azoospermia factor region deletions in infertile male subjects among Malaysians
Author(s) -
Hussein A. A.,
Vasudevan R.,
Patimah I.,
Prashant N.,
Nora F. A.
Publication year - 2015
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/and.12240
Subject(s) - azoospermia factor , male infertility , high resolution melt , y chromosome , biology , infertility , genetics , azoospermia , polymerase chain reaction , andrology , gene , medicine , pregnancy
Summary Azoospermia factor region ( AZF ) deletions ( AZF a, AZF b, AZF c and AZF d) in the Y chromosome were analysed in male infertility subjects in various populations with conflicting results. This study comprised of 54 infertile males and 63 fertile controls, and the frequency of AZF a, AZF b, AZF c and AZF d deletions were determined using conventional polymerase chain reaction ( PCR ) as well as real‐time PCR ‐high resolution melting analysis‐based methods. The results of this study showed that, three of 54 cases (5.55%) had AZF (a, b and c) deletions (two had AZF c and one had AZF a deletions). Four cases were found to have AZF d deletions (7.4%) with two of them being associated with AZF c deletions ( P = 0.028). The frequency of AZF (a, b and c) deletions in Malaysian infertile male subjects was found to be comparable with other populations. AZF d deletions were found to be significant ( P < 0.05) in male infertility and it may be associated with other types of AZF deletions.