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Human catalase gene polymorphism ( CAT C ‐262 T ) and risk of male infertility
Author(s) -
Sabouhi S.,
Salehi Z.,
Bahadori M. H.,
Mahdavi M.
Publication year - 2015
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/and.12228
Subject(s) - genotype , catalase , infertility , male infertility , allele , pathogenesis , biology , gene polymorphism , polymorphism (computer science) , medicine , andrology , genetics , endocrinology , immunology , gene , oxidative stress , pregnancy
Summary Infertility is the failure of a couple to engender after endeavouring at least one full year of unprotected intercourse. It has been reported that reactive oxygen species contributed to pathogenesis of various disease. To inactivate ROS cells biosynthesise several antioxidant enzymes, one of them is catalase which contributes H 2 O 2 to H 2 O and O 2 . This study set out to delineate the association of catalase C ‐262 T polymorphism with idiopathic male infertility. The study included 195 men with idiopathic infertility and 190 healthy volunteers. Genomic DNA was extracted from peripheral blood leucocytes. Genotype and allele frequencies were determined in patients and controls using allele‐specific PCR ( AS ‐ PCR ). The prevalence of genotype frequencies of the CAT CC / CT / TT was 31.79%, 65.12% and 3.07%, respectively, in infertile subjects, as against 24.73%, 55.26% and 20%, respectively, in healthy volunteers. Statistical analysis has emerged significant difference from the comparison of either genotype ( P < 0.05 ). Taking into accounts of results, the catalase C ‐262 T polymorphism indicates that CAT ‐262 T / T genotype confers less susceptibility to male infertility. Further studies with larger numbers of patients are required for further evaluation and confirmation of our finding.