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Regeneration of spermatogenesis in a mouse model of azoospermia by follicle‐stimulating hormone and oestradiol
Author(s) -
Jafarian A.,
Sadeghi M. R.,
Pejhan N.,
Salehkhou S.,
Lakpour N.,
Akhondi M. M.
Publication year - 2014
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/and.12198
Subject(s) - spermatogenesis , azoospermia , medicine , endocrinology , biology , andrology , cyclin b1 , busulfan , sertoli cell , follicle stimulating hormone , testosterone (patch) , testicular atrophy , hormone , stem cell , luteinizing hormone , cell cycle , microbiology and biotechnology , infertility , cancer , pregnancy , cyclin dependent kinase 1 , genetics , hematopoietic stem cell transplantation
Summary Busulfan is a chemotherapeutic drug that induces sterility, azoospermia and testicular atrophy. To induce degeneration of spermatogenesis, we used different amounts of busulfan. Adult male C 57 B l/6 mice were treated with 15, 30 and 45 mg kg −1 of busulfan. After 5 weeks, animals had daily injections of 7.5 IU human follicle‐stimulating hormone (h FSH ) and 12.5 μg kg −1 oestradiol benzoate ( EB ), separately or simultaneously. After this time, the animals were killed and blood samples were taken through cardiac puncture. Testes were used for histopathology experiments, DNA flow cytometry and RNA extraction for expression of c‐kit and cyclin B 1 genes. EB unlike FSH has induced stimulatory effects on spermatogenesis, increased the level of serum testosterone 2‐fold and caused a 2‐fold increase in the number of haploid cells. The result showed that h FSH with EB multiplied EB stimulatory effects on spermatogenesis up to four times. Expression of c‐kit and cyclin B 1 genes increased in EB and h FSH + EB groups. These findings suggest that EB regulates spermatogonial stem cells via h FSH . h FSH with EB had synergistic effect on regeneration of spermatogenesis.