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Semen analysis of G olden R etriever healthy dogs and those affected by muscular dystrophy
Author(s) -
Peres M. A.,
Rocha A. M.,
Vannucchi C. I.,
Mendes C. M.,
Cavalcanti P. V.,
Nichi M.,
Ambrosio C. E.,
Miglino M. A.,
Visintin J. A.,
D'Ávila Assumpção M. E. O.
Publication year - 2014
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/and.12079
Subject(s) - semen , acrosome , andrology , sperm , dna fragmentation , biology , breed , semen analysis , labrador retriever , anatomy , medicine , pathology , zoology , genetics , infertility , apoptosis , pregnancy , programmed cell death
Summary This study aimed to characterise canine flow cytometry semen analysis, as well as seminal reactive oxygen species dosage using the G olden R etriever breed as model of study. Moreover, we searched for the influence of muscular dystrophy in G olden R etriever dogs on semen parameters. Thirty‐seven semen samples were obtained from healthy G olden R etrievers ( n = 15) and from muscular dystrophy affected dogs ( n = 22). Sperm‐rich fractions were analysed by standardised breeding soundness examination in addition to the assay of fluorescence assisted cell sorting for acrosome integrity, mitochondrial activity and DNA fragmentation. Volume of ejaculate, per cent of motile spermatozoa and vigour were similar between groups; there were no differences in the per cent of minor and major defects. Integrity of acrosomal membrane, mitochondrial potential and sperm DNA fragmentation had no significant differences between groups either. Animals from control group had higher concentration of spontaneous seminal oxidative species in comparison with affected animals. Dogs affected by dystrophy had seminal parameters similar to those observed in healthy dogs except for the lower concentration of oxidative species. Future studies aiming to establish reference values for canine seminal parameters should be considered preferably with distinction of breeds.