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4977‐bp mitochondrial DNA deletion in infertile patients with varicocele
Author(s) -
Gashti N. G.,
Salehi Z.,
Madani A. H.,
Dalivandan S. T.
Publication year - 2014
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/and.12073
Subject(s) - varicocele , male infertility , semen , infertility , andrology , population , biology , semen analysis , mitochondrial dna , spermatic cord , medicine , anatomy , genetics , pregnancy , gene , environmental health
Summary Varicocele is the abnormal inflexion and distension of veins of the pampiniform plexus within spermatic cord and is one of the amendable causes of male infertility. It can increase reactive oxygen species ( ROS ) production in semen and cause oxidative stress. The purpose of this study was to analyse spermatozoa mt DNA 4977‐bp deletion in infertile men with varicocele. To detect 4977‐bp deletion in spermatozoa mt DNA , semen samples of 60 infertile patients with clinical varicocele and 90 normal men from northern Iran were prepared. After extraction of spermatozoa total DNA , G ap polymerase chain reaction ( G ap PCR ) was performed. 4977‐bp deletion was observed in 81.66% of patients with varicocele, while approximately 15.55% of controls had this deletion. As spermatozoa from patients with varicocele had a high frequency of occurrence of 4977‐bp deletion in mt DNA [ OR = 24.18, 95% confidence interval ( CI ) = 10.15–57.57, P < 0.0001], varicocele may induce mt DNA deletion in spermatozoa and cause infertility in north I ranian men. However, to determine the relation between sperm mt DNA 4977‐bp deletion and varicocele‐induced infertility, larger population‐based studies are needed. It is concluded that there is an association between sperm mt DNA 4977‐bp deletion and varicocele‐induced infertility in the population studied.