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Infertility in rats subjected to genitofemoral nerve section is not associated with testicular damage
Author(s) -
GutiérrezPérez O.,
RojasCastañeda J. C.,
ChavezSaldaña M.,
Reyes G.,
ViguerasVillaseñor R. M.
Publication year - 2014
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/and.12063
Subject(s) - gubernaculum , cremaster muscle , infertility , inguinal canal , testicle , male infertility , epididymis , scrotum , sperm , spermatogenesis , medicine , nerve injury , seminiferous tubule , biology , andrology , anatomy , microcirculation , surgery , sertoli cell , hernia , pregnancy , inguinal hernia , genetics
Summary This work was aimed at assessing the relationship between testicular ascent and infertility induced by genitofemoral nerve ( GFN ) section in rats. Eighteen male rats were assigned to three experimental groups as follows: (i) Group SGFN was subjected to surgical section of genitofemoral nerve; (ii) Group Sham; (iii) Control group. The GFN was cut at puberty (28D), and the contralateral testis removed at 90D, with fertility tests at 120D. At 150D, maturity index, epithelial area and histopathological index of seminiferous tubules of all rats were determined and statistically compared between superior and inferior testicle poles, and between groups. There were no differences in testicular parameters, sperm morphology or sperm concentrations ( P > 0.05). Section of NGF interfered with fertility (58.3 ± 15.4 in SGFN versus 83.3 ± 10.5 in Sham) and litter size (6.2 ± 1.1 in SGFN versus 10.7 ± 1.4 in Sham). Cremaster of SGFN group showed early neuropathy. The GFN section induced partial testicular ascent and diminished fertility without damage on testicular morphology or spermatic parameters, because, cremaster could affect the contractibility and ejaculation mechanisms in which it participates. The study of the damage on cremaster induced by an injury on GFN could have an overview of the mechanisms inherent in the testicular ascent induced by this iatrogenic alteration and their potential risks on fertility.