Premium
Effects of montelukast and zileuton on testicular torsion/detorsion injury in rats
Author(s) -
Isikdemir F.,
Kurcer Z.,
Dengiz G. O.,
Sipahi E. Y.,
Banoglu Z. N.,
Baba F.,
Acikgoz S.,
Kelek S.
Publication year - 2014
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/and.12042
Subject(s) - zileuton , montelukast , testicular torsion , xylazine , spermatic cord torsion , ketamine hydrochloride , medicine , antagonist , malondialdehyde , leukotriene , leukotriene receptor , anesthesia , pharmacology , endocrinology , chemistry , surgery , oxidative stress , receptor , ketamine , arachidonic acid , arachidonate 5 lipoxygenase , biochemistry , asthma , enzyme
Summary The aim of this study was to evaluate and compare the effects of 5‐lipoxygenase enzyme (5‐ LO ) inhibitor zileuton and cysteinyl leukotriene receptor (Cys LT 1R) antagonist montelukast in testicular torsion/detorsion (T/D) injury model in rats. Rats were anaesthetised with 75 mg kg −1 ketamine hydrochloride and 8 mg kg −1 xylazine intraperitoneal before the operation. Torsion was created by rotating the right testis 720° clockwise and maintained by fixing the testis. The rats were treated with Cys LT 1R antagonist montelukast (10 mg kg −1 ; i.p.), 5‐ LO inhibitor zileuton (3 mg kg −1 ; i.p.), and vehicle, at 30 min prior detorsion. After 1 h of torsion, the testis was counter‐rotated to the natural position and replaced into the scrotum. Malondialdehyde ( MDA ) level was measured in testicular tissue after 3 h of reperfusion. Histological examination was performed after 24 h of reperfusion. T/D caused a significant increase in MDA level and histopathological injury in testes. Montelukast and zileuton treatments prevented the T/D‐induced augmentation in MDA levels. Only zileuton treatment significantly reduced the T/D‐induced histopathological injury. In this study, we demonstrated for the first time that zileuton had protective effects on testicular T/D injury. We have also found that zileuton is more effective than montelukast on histopathological injury.