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Immune changes beyond Th2 pathways during rapid multifood immunotherapy enabled with omalizumab
Author(s) -
Manohar Monali,
Dunham Diane,
Gupta Sheena,
Yan Zheng,
Zhang Wenming,
Minnicozzi Samantha,
Kirkey Matthew,
Bunning Bryan,
Roy Chowdhury Roshni,
Galli Stephen J.,
Boyd Scott D.,
Kost Laurie Elizabeth,
Chinthrajah R. Sharon,
Desai Manisha,
Oettgen Hans C.,
Maecker Holden T.,
Yu Wong,
DeKruyff Rosemarie H.,
Andorf Sandra,
Nadeau Kari C.
Publication year - 2021
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/all.14833
Subject(s) - omalizumab , immunotherapy , medicine , immune system , immunology , immunoglobulin e , antibody
Background Multifood oral immunotherapy (mOIT) with adjunctive anti‐IgE (omalizumab, XOLAIR ® ) treatment affords safe, effective, and rapid desensitization to multiple foods, although the specific immune mechanisms mediating this desensitization remain to be fully elucidated. Methods Participants in our phase 2 mOIT trial (NCT02643862) received omalizumab from baseline to week 16 and mOIT from week 8 to week 36. We compared the immune profile of PBMCs and plasma taken at baseline, week 8, and week 36 using high‐dimensional mass cytometry, component‐resolved diagnostics, the indirect basophil activation test, and Luminex. Results We found (i) decreased frequency of IL‐4 + peanut‐reactive CD4 + T cells and a marked downregulation of GPR15 expression and CXCR3 frequency among γδ and CD8 + T‐cell subsets at week 8 during the initial, omalizumab‐alone induction phase; (ii) significant upregulation of the skin‐homing receptor CCR4 in peanut‐reactive CD4 + T and Th2 effector memory (EM) cells and of cutaneous lymphocyte‐associated antigen (CLA) in peanut‐reactive CD8 + T and CD8 + EM cells; (iii) downregulation of CD86 expression among antigen‐presenting cell subsets; and (iv) reduction in pro‐inflammatory cytokines, notably IL‐17, at week 36 post‐OIT. We also observed significant attenuation of the Th2 phenotype post‐OIT, defined by downregulation of IL‐4 peanut‐reactive T cells and OX40 in Th2EM cells, increased allergen component‐specific IgG4/IgE ratio, and decreased allergen‐driven activation of indirectly sensitized basophils. Conclusions This exploratory study provides novel comprehensive insight into the immune underpinnings of desensitization through omalizumab‐facilitated mOIT. Moreover, this study provides encouraging results to support the complex immune changes that can be induced by OIT.