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Comparative analysis of the role of mast cells in murine asthma models using Kit‐sufficient mast cell‐deficient animals
Author(s) -
Pohlmeier Lea,
Sonar Sanchaita Sriwal,
Rodewald HansReimer,
Kopf Manfred,
Tortola Luigi
Publication year - 2021
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/all.14765
Subject(s) - immunology , asthma , ovalbumin , medicine , sensitization , mucus , allergy , house dust mite , immunoglobulin e , mast cell , inflammation , cytokine , allergic inflammation , airway , allergen , immune system , antibody , biology , ecology , surgery
Background Asthma is a frequent chronic disease that can potentially severely affect the respiratory capacity and well‐being of patients. Mast cells (MCs) are regarded as major players in human asthma due to their capacity to release crucial inflammatory mediators following allergen exposure. However, unambiguous characterization of their role in animal models has long been hindered by the unavailability of specific MC‐deficient models lacking confounding MC‐unrelated effects. This study aims to examine the role of MCs in Kit ‐sufficient MC‐deficient Cpa3 Cre /+ mice. Methods We used a variety of models of acute and chronic asthma employing distinct routes and regimes of sensitization. These sensitizations were done via the peritoneal cavity, the skin, or the lung. Additionally, different allergens, i.e. ovalbumin and house dust mite extract, were used. Results Our results show that the absence of MCs had no impact on the severity of allergic airway inflammation in any of the tested mouse models, as measured by leukocyte infiltration in the airways, cytokine expression, antibody production, airway hyper‐responsiveness and mucus production. Conclusion This indicates that MCs do not play a major role in murine allergic airway inflammation.