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Temporal and long‐term gut microbiota variation in allergic disease: A prospective study from infancy to school age
Author(s) -
Simonyté Sjödin K.,
Hammarström M.L.,
Rydén P.,
Sjödin A.,
Hernell O.,
Engstrand L.,
West C. E.
Publication year - 2019
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/all.13485
Subject(s) - prospective cohort study , medicine , variation (astronomy) , immunology , gut flora , term (time) , disease , pediatrics , physics , quantum mechanics , astrophysics
Background Compositional changes in the early‐life gut microbiota have been implicated in IgE‐associated allergic diseases, but there is lack of longitudinal studies. We examined gut microbiota development from infancy to school age in relation to onset of IgE‐associated allergic diseases. At 8 years of age, we also examined the relationship between gut microbiota and T‐cell regulation, estimated as responses to polyclonal T‐cell activation. Methods Stool samples were collected from 93 children at 4, 6, 13 months, and 8 years of age. The gut microbiota was profiled using 16S rRNA gene sequencing. Peripheral blood was drawn from all children, and mononuclear cells were polyclonally activated. Levels of IL ‐10 and FOXP 3 mRNA copies were determined using real‐time quantitative reverse transcriptase‐PCR. Results At 8 years of age, 21 children were diagnosed with IgE‐associated allergic disease and 90% displayed allergic comorbidity. Seventy‐two children were nonallergic and nonsensitized. Statistical tests with multiple testing corrections demonstrated temporal underrepresentation of Ruminococcus and consistent underrepresentation of Bacteroides, Prevotella, and Coprococcus in allergic compared to nonallergic children from infancy to school age. The gut microbiota of the allergic 8‐year‐olds was enriched in Bifidobacterium and depleted of Lactobacillus, Enterococcus, and Lachnospira . In allergic 8‐year‐olds, Faecalibacterium correlated with IL ‐10 mRNA levels ( r s = 0.49 , P adj = 0.02) with the same trend for FOXP 3 ( r s = 0.39 , P adj = 0.08). Conclusions We identified both temporal and long‐term variation in the differential abundance of specific bacterial genera in children developing IgE‐associated allergic disease. Improved dietary interventions aiming at expanding immune‐modulatory taxa could be studied for prevention of allergic disease.