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Altered miR‐193a‐5p expression in children with cow's milk allergy
Author(s) -
D'Argenio V.,
Del Monaco V.,
Paparo L.,
De Palma F. D. E.,
Nocerino R.,
D'Alessio F.,
Visconte F.,
Discepolo V.,
Del Vecchio L.,
Salvatore F.,
Berni Canani R.
Publication year - 2018
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/all.13299
Subject(s) - pathogenesis , downregulation and upregulation , allergy , peripheral blood mononuclear cell , foxp3 , epigenetics , microrna , milk allergy , immunology , dna methylation , medicine , food allergy , biology , gene expression , gene , genetics , immune system , in vitro
Background Cow's milk allergy ( CMA ) is one of the most common food allergies in children. Epigenetic mechanisms have been suggested to play a role in CMA pathogenesis. We have shown that DNA methylation of Th1/Th2 cytokine genes and FoxP3 affects CMA disease course. Preliminary evidence suggests that also the mi RN ome could be implicated in the pathogenesis of allergy. Main study outcome was to comparatively evaluate mi RN ome in children with CMA and in healthy controls. Methods Peripheral blood mononuclear cells were obtained from children aged 4‐18 months: 10 CMA patients, 9 CMA patients who outgrew CMA , and 11 healthy controls. Small RNA libraries were sequenced using a next‐generation sequencing‐based approach. Functional assessment of IL ‐4 expression was also performed. Results Among the mi RNA s differently expressed, 2 were upregulated and 14 were downregulated in children with active CMA compared to healthy controls. miR‐193a‐5p resulted the most downregulated mi RNA in children with active CMA compared to healthy controls. The predicted targets of miR‐193a‐5p resulted upregulated in CMA patients compared to healthy controls. Peripheral blood CD 4 + T cells transfected with a miR193a‐5 inhibitor showed a significant upregulation of IL ‐4 mRNA and its protein expression. Children who outgrew CMA showed mi RNA ‐193a‐5p level, and its related targets expression, similar to that observed in healthy controls. Conclusions Our results suggest that miR‐193a‐5p is a post‐transcriptional regulator of IL ‐4 expression and could have a role in IgE‐mediated CMA . This mi RNA could be a novel diagnostic and therapeutic target for this common form of food allergy in childhood.