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Forkhead box protein‐3 (Foxp3)‐producing dendritic cells suppress allergic response
Author(s) -
Liu X.Y.,
Xu L.Z.,
Luo X.Q.,
Geng X.R.,
Liu Z.Q.,
Yang L.T.,
Yang G.,
Chen S.,
Liu Z.G.,
Li H.B.,
Yang L.T.,
Luan T.G.,
Yang P.C.
Publication year - 2017
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/all.13088
Subject(s) - foxp3 , forkhead transcription factors , immunology , dendritic cell , biology , immune system , biochemistry , regulation of gene expression , gene
Background The generation of the tolerogenic dendritic cells ( DC ) is not fully understood yet. Forkhead box protein‐3 (Foxp3) is an important molecule in the immune tolerance. This study tests a hypothesis that DC s express Foxp3, which can be upregulated by Staphylococcal enterotoxin B ( SEB ). Methods The expression of Foxp3 by DC s was evaluated by real‐time RT ‐ PCR , Western blotting, flow cytometry, and chromatin immunoprecipitation assay. Results We observed that mice treated with SEB at 0.25–0.5 μg/mouse showed high frequencies of transforming growth factor ( TGF )‐β‐producing CD 4 + T cells and TGF ‐β‐producing DC s in the intestine, while the IL ‐4 + CD 4 + T cells and TIM 4 + DC s were dominated in the intestine in mice treated with SEB at 1–10 μg/mouse. Treating DC s with SEB in the culture induced high levels of Foxp3 at the TGF ‐β promoter locus. The function of Foxp3 was blocked by STAT 6 (signal transducer and activator transcription‐6); the latter was induced by exposing DC s to SEB in the culture at doses of 100–400 ng/ml. Treating allergic mice with specific immunotherapy ( SIT ) together with SEB significantly promoted the therapeutic effects on the allergic responses than treating with SIT alone. Conclusion Dendritic cells have the capacity to express Foxp3, which can be upregulated by exposure to SEB .