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CD 48 on blood leukocytes and in serum of asthma patients varies with severity
Author(s) -
Gangwar R. S.,
MinaiFleminger Y.,
Seaf M.,
Gutgold A.,
Shikotra A.,
Barber C.,
Chauhan A.,
Holgate S.,
Bradding P.,
Howarth P.,
Eliashar R.,
Berkman N.,
LeviSchaffer F.
Publication year - 2017
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/all.13082
Subject(s) - asthma , eosinophil , immunology , medicine , proinflammatory cytokine , mast cell , allergy , inflammation
Background CD 48 is a membrane receptor ( mCD 48) on eosinophils and mast cells and exists in a soluble form ( sCD 48). CD 48 has a pivotal role in murine asthma and in the proinflammatory interactions of mast cells with eosinophils via its ligand CD 244. Thus, CD 48 might be important in human asthma. Methods Therefore, two separate cohorts ( IL and UK ) comprising mild, moderate, and severe asthma and healthy volunteers were evaluated for blood leukocyte mCD 48 expression and sCD 48 in serum. Asthmatic bronchial biopsies were immunostained for CD 48. sCD 48 effect on CD 244‐dependent eosinophil activation was evaluated. Results Eosinophil mCD 48 expression was significantly elevated in moderate while downregulated in severe asthma. mCD 48 expression on B, T, and NK cells and monocytes in severe asthma was significantly increased. sCD 48 levels were significantly higher in mild while reduced in severe asthma. sCD 48 optimal cutoff values for differentiating asthma from health were identified as >1482 pg/ml ( IL ) and >1619 pg/ml ( UK ). In asthmatic bronchial biopsies, mCD 48 was expressed predominantly by eosinophils. sCD 48 inhibited anti‐ CD 244‐induced eosinophil activation. Conclusions mCD 48 and sCD 48 are differentially expressed in the peripheral blood of asthma patients of varying severity. sCD 48 inhibits CD 244‐mediated eosinophil activation. These findings suggest that CD 48 may play an important role in human asthma.