Premium
Transcriptomic/proteomic identification of allergens in the mite Tyrophagus putrescentiae
Author(s) -
Cui Y.,
Yu L.,
Teng F.,
Zhang C.,
Wang N.,
Yang L.,
Zhou Y.
Publication year - 2016
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/all.12999
Subject(s) - tyrophagus putrescentiae , allergen , biology , food allergens , acaridae , mite , proteome , recombinant dna , transcriptome , immunoglobulin e , microbiology and biotechnology , immunology , allergy , antibody , acariformes , gene , genetics , botany , gene expression
While a number of allergens from house dust mites have been described, much remains to be discovered about allergens from storage mites. Here, next‐generation sequencing was combined with MS / MS shotgun proteomics to identify proteins, especially potential allergens from Tyrophagus putrescentiae , commonly found in stored food products, especially flour. cDNA s of suspected allergens were cloned and expressed from bacterial cells, and recombinant allergens were tested for binding to IgE in sera from T. putrescentiae ‐sensitive patients. These analyses identified three previously uncharacterized allergens, Tyr p 28, Tyr p 35, and Tyr p 36, which have been officially assigned by the WHO / IUIS Allergen Nomenclature Sub‐committee. Recombinant proteins rT yr p 28, rT yr p 35, and rT yr p 36 bound with 47.1%, 82.4%, and 70.6% of T. putrescentiae ‐sensitive sera. We provide here a new method to identify allergens by the combination of transcriptomic and proteomic approaches.