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Protein profiles of CCL 5, HPGDS , and NPSR 1 in plasma reveal association with childhood asthma
Author(s) -
Hamsten C.,
Häggmark A.,
Grundström J.,
Mikus M.,
Lindskog C.,
Konradsen J. R.,
Eklund A.,
Pershagen G.,
Wickman M.,
Grunewald J.,
Melén E.,
Hedlin G.,
Nilsson P.,
Hage M.
Publication year - 2016
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/all.12927
Subject(s) - asthma , immunology , medicine , receptor , endocrinology
Asthma is a common chronic childhood disease with many different phenotypes that need to be identified. We analyzed a broad range of plasma proteins in children with well‐characterized asthma phenotypes to identify potential markers of childhood asthma. Using an affinity proteomics approach, plasma levels of 362 proteins covered by antibodies from the Human Protein Atlas were investigated in a total of 154 children with persistent or intermittent asthma and controls. After screening, chemokine ligand 5 ( CCL 5) hematopoietic prostaglandin D synthase ( HPGDS ) and neuropeptide S receptor 1 ( NPSR 1) were selected for further investigation. Significantly lower levels of both CCL 5 and HPGDS were found in children with persistent asthma, while NPSR 1 was found at higher levels in children with mild intermittent asthma compared to healthy controls. In addition, the protein levels were investigated in another respiratory disease, sarcoidosis, showing significantly higher NPSR 1 levels in sera from sarcoidosis patients compared to healthy controls. Immunohistochemical staining of healthy tissues revealed high cytoplasmic expression of HPGDS in mast cells, present in stroma of both airway epithelia, lung as well as in other organs. High expression of NPSR 1 was observed in neuroendocrine tissues, while no expression was observed in airway epithelia or lung. In conclusion, we have utilized a broad‐scaled affinity proteomics approach to identify three proteins with altered plasma levels in asthmatic children, representing one of the first evaluations of HPGDS and NPSR 1 protein levels in plasma.