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Decreased expression of ectonucleotidase E‐ NPP 1 in leukocytes from subjects with severe asthma exacerbation
Author(s) -
Montaño L. M.,
Vargas M. H.,
DíazHernández V.,
De Ita M.,
Kazakova R.,
BarajasLópez C.
Publication year - 2016
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/all.12772
Subject(s) - exacerbation , asthma , medicine , pathophysiology , immunology , immunofluorescence , asthma exacerbations , antibody
Several studies suggest that ATP and related nucleotides play a role in the pathophysiology of asthma. However, the functionality of ectonucleotidases in this disease has been scantly investigated. We studied total ectonucleotidase activity in leukocytes from patients suffering from asthma exacerbation and explored the expression of E‐ NTPD ase 1, 2, 3, and 8, and E‐ NPP 1, 2, and 3, in their polymorphonuclear cells by immunofluorescence and qPCR . Leukocytes from patients with mild or moderate asthma exacerbation had similar ectonucleotidase activity than leukocytes from healthy subjects, while in patients with severe asthma exacerbation, this activity was lower. Of the ectonucleotidases studied, only E‐ NPP 1 displayed diminished immunofluorescence and a significant decrease in its mRNA expression, both in patients with severe asthma exacerbation. This reduced E‐ NPP 1 expression could be responsible for increased amounts of ATP or other nucleotides, capable of worsening asthma exacerbation, and warranting further investigation.