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Effector cell signature in peripheral blood following nasal allergen challenge in grass pollen allergic individuals
Author(s) -
Shamji M. H.,
Bellido V.,
Scadding G. W.,
Layhadi J. A.,
Cheung D. K. M.,
Calderon M. A.,
Asare A.,
Gao Z.,
Turka L. A.,
Tchao N.,
Togias A.,
Phippard D.,
Durham S. R.
Publication year - 2015
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/all.12543
Subject(s) - immunology , cd80 , cd86 , dendritic cell , basophil activation , basophil , t cell , flow cytometry , il 2 receptor , cd63 , medicine , biology , antigen , immunoglobulin e , cd40 , cytotoxic t cell , immune system , in vitro , antibody , microvesicles , gene , microrna , biochemistry
Background Several studies have demonstrated the time course of inflammatory mediators in nasal fluids following nasal allergen challenge ( NAC ), whereas the effects of NAC on cells in the periphery are unknown. We examined the time course of effector cell markers (for basophils, dendritic cells and T cells) in peripheral blood after nasal grass pollen allergen challenge. Methods Twelve participants with seasonal allergic rhinitis underwent a control (diluent) challenge followed by NAC after an interval of 14 days. Nasal symptoms and peak nasal inspiratory flow ( PNIF ) were recorded along with peripheral basophil, T ‐cell and dendritic cell responses (flow cytometry), T ‐cell proliferative responses (thymidine incorporation), and cytokine expression ( F luoro S pot assay). Results Robust increases in nasal symptoms and decreases in PNIF were observed during the early (0–1 h) response and modest significant changes during the late (1–24 h) response. Sequential peaks in peripheral blood basophil activation markers were observed ( CD 107a at 3 h, CD 63 at 6 h, and CD 203c bright at 24 h). T effector/memory cells ( CD 4 + CD 25 lo ) were increased at 6 h and accompanied by increases in CD 80 + and CD 86 + plasmacytoid dendritic cells (p DC s). Ex vivo grass antigen‐driven T ‐cell proliferative responses and the frequency of IL ‐4 + CD 4 + T cells were significantly increased at 6 h after NAC when compared to the control day. Conclusion Basophil, T ‐cell, and dendritic cell activation increased the frequency of allergen‐driven IL ‐4 + CD 4 + T cells, and T ‐cell proliferative responses are detectable in the periphery after NAC . These data confirm systemic cellular activation following a local nasal provocation.