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Extracellular DNA traps in bronchoalveolar fluid from a murine eosinophilic pulmonary response
Author(s) -
Cunha A. A.,
Porto B. N.,
Nuñez N. K.,
Souza R. G.,
Vargas M. H. M.,
Silveira J. S.,
Souza T. T. R.,
Jaeger N.,
Pitrez P. M.
Publication year - 2014
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/all.12507
Subject(s) - bronchoalveolar lavage , eosinophil , neutrophil extracellular traps , eosinophil peroxidase , extracellular , ovalbumin , lung , immunology , extracellular fluid , pathology , chemistry , biology , inflammation , medicine , asthma , microbiology and biotechnology , immune system
Asthma is associated with a loss of the structural integrity of airway epithelium and dysfunction of the physical barrier, which protects airways from external harmful factors. Granulocyte activation causes the formation of extracellular traps, releasing web‐like structures of DNA and proteins, being important to kill pathogens extracellularly. We investigated whether eosinophils infiltrating airways in an experimental model of asthma would induce eosinophil extracellular traps ( EET s) in bronchoalveolar lavage fluid and lung tissue. We showed that an ovalbumin ( OVA ) asthma protocol presented a significant increase in eosinophil counts with increased extracellular DNA in bronchoalveolar lavage fluid as well as in lung tissue, confirming the presence of DNA traps colocalized with eosinophil peroxidase. EET s formation was reversed by DN ase treatment. With these approaches, we demonstrated for the first time that OVA ‐challenged mice release extracellular DNA traps, which could aggravate pulmonary dysfunction.