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Identification of Maillard reaction products on peanut allergens that influence binding to the receptor for advanced glycation end products
Author(s) -
Mueller G. A.,
Maleki S. J.,
Johnson K.,
Hurlburt B. K.,
Cheng H.,
Ruan S.,
Nesbit J. B.,
Pomés A.,
Edwards L. L.,
Schorzman A.,
Deterding L. J.,
Park H.,
Tomer K. B.,
London R. E.,
Williams J. G.
Publication year - 2013
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/all.12261
Subject(s) - maillard reaction , glycation , chemistry , amadori rearrangement , receptor , identification (biology) , biochemistry , allergen , advanced glycation end product , immunology , food science , medicine , allergy , biology , botany
Background Recent immunological data demonstrated that dendritic cells preferentially recognize advanced glycation end product ( AGE )‐modified proteins, upregulate expression of the receptor for AGE ( RAGE ), and consequently bias the immune response toward allergy. Methods Peanut extract was characterized by mass spectrometry ( MS ) to elucidate the specific residues and specific AGE modifications found in raw and roasted peanuts and on rA ra h 1 that was artificially glycated by incubation with glucose or xylose. The binding of the RAGE ‐V1C1 domain to peanut allergens was assessed by PAGE and Western analysis with anti‐Ara h 1, 2, and 3 antibodies. IgE binding to rA ra h 1 was also assessed using the same methods. Results AGE modifications were found on Ara h 1 and Ara h 3 in both raw and roasted peanut extract. No AGE modifications were found on Ara h 2. Mass spectrometry and Western blot analysis demonstrated that RAGE binds selectively to Ara h 1 and Ara h 3 derived from peanut extract, whereas the analysis failed to demonstrate Ara h 2 binding to RAGE . rA ra h 1 with no AGE modifications did not bind RAGE ; however, after AGE modification with xylose, rA ra h 1 bound to RAGE . Conclusions AGE modifications to Ara h 1 and Ara h 3 can be found in both raw and roasted peanuts. Receptor for AGE was demonstrated to selectively interact with AGE ‐modified rA ra h 1. If sensitization to peanut allergens occurs in dendritic cells via RAGE interactions, these cells are likely interacting with modified Ara h 1 and Ara h 3, but not Ara h 2.