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Tolerogenic CX3CR1 + B cells suppress food allergy‐induced intestinal inflammation in mice
Author(s) -
Liu Z.Q.,
Wu Y.,
Song J.P.,
Liu X.,
Liu Z.,
Zheng P.Y.,
Yang P.C.
Publication year - 2013
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/all.12218
Background B lymphocytes are an important cell population of the immune regulation; their role in the regulation of food allergy has not been fully understood yet. Objective This study aims to investigate the role of a subpopulation of tolerogenic B cells (Tol BC ) in the generation of regulatory T cells (Treg) and in the suppression of food allergy‐induced intestinal inflammation in mice. Methods The intestinal mucosa‐derived CD 5 + CD 19 + CX 3 CR 1 + Tol BC s were characterized by flow cytometry; a mouse model of intestinal T helper (Th)2 inflammation was established to assess the immune regulatory role of this subpopulation of Tol BC s. Results A subpopulation of CD 5 + CD 19 + CX 3 CR 1 + B cells was detected in the mouse intestinal mucosa. The cells also expressed transforming growth factor ( TGF )‐β and carried integrin alpha v beta 6 (αvβ6). Exposure to recombinant αvβ6 and anti‐IgM antibody induced naive B cells to differentiate into the TGF ‐β‐producing Tol BC s. Coculturing this subpopulation of Tol BC s with Th0 cells generated CD 4 + CD 25 + Foxp3 + Tregs. Adoptive transfer with the Tol BC s markedly suppressed the food allergy‐induced intestinal Th2 pattern inflammation in mice. Conclusions CD 5 + CD 19 + CX 3 CR 1 + Tol BC s are capable of inducing Tregs in the intestine and suppress food allergy‐related Th2 pattern inflammation in mice.