z-logo
Premium
Tolerogenic CX3CR1 + B cells suppress food allergy‐induced intestinal inflammation in mice
Author(s) -
Liu Z.Q.,
Wu Y.,
Song J.P.,
Liu X.,
Liu Z.,
Zheng P.Y.,
Yang P.C.
Publication year - 2013
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/all.12218
Background B lymphocytes are an important cell population of the immune regulation; their role in the regulation of food allergy has not been fully understood yet. Objective This study aims to investigate the role of a subpopulation of tolerogenic B cells (Tol BC ) in the generation of regulatory T cells (Treg) and in the suppression of food allergy‐induced intestinal inflammation in mice. Methods The intestinal mucosa‐derived CD 5 + CD 19 + CX 3 CR 1 + Tol BC s were characterized by flow cytometry; a mouse model of intestinal T helper (Th)2 inflammation was established to assess the immune regulatory role of this subpopulation of Tol BC s. Results A subpopulation of CD 5 + CD 19 + CX 3 CR 1 + B cells was detected in the mouse intestinal mucosa. The cells also expressed transforming growth factor ( TGF )‐β and carried integrin alpha v beta 6 (αvβ6). Exposure to recombinant αvβ6 and anti‐IgM antibody induced naive B cells to differentiate into the TGF ‐β‐producing Tol BC s. Coculturing this subpopulation of Tol BC s with Th0 cells generated CD 4 + CD 25 + Foxp3 + Tregs. Adoptive transfer with the Tol BC s markedly suppressed the food allergy‐induced intestinal Th2 pattern inflammation in mice. Conclusions CD 5 + CD 19 + CX 3 CR 1 + Tol BC s are capable of inducing Tregs in the intestine and suppress food allergy‐related Th2 pattern inflammation in mice.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here