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Cinnamon extract inhibits degranulation and de novo synthesis of inflammatory mediators in mast cells
Author(s) -
Hagenlocher Y.,
Bergheim I.,
Zacheja S.,
Schäffer M.,
Bischoff S. C.,
Lorentz A.
Publication year - 2013
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/all.12122
Subject(s) - degranulation , mast cell , in vivo , tryptase , p38 mitogen activated protein kinases , histamine , proteases , chemistry , in vitro , pharmacology , signal transduction , immunology , microbiology and biotechnology , mapk/erk pathway , biology , biochemistry , receptor , enzyme
Abstract Background Mast cells ( MC ) are main effector cells of allergic and other inflammatory reactions; however, only a few anti‐ MC agents are available for therapy. It has been reported that cinnamon extract ( CE ) attenuates allergic symptoms by affecting immune cells; however, its influence on MC was not studied so far. Here, we analyzed the effects of CE on human and rodent MC in vitro and in vivo . Methods Expression of MC ‐specific proteases was examined in vivo in duodenum of mice following oral administration of CE . Release of mediators and phosphorylation of signaling molecules were analyzed in vitro in human MC isolated from intestinal tissue (hi MC ) or RBL ‐2H3 cells challenged with CE prior to stimulation by Fcε RI cross‐linking. Results Following oral treatment with CE , expression of the mast cell proteases MCP 6 and MC ‐ CPA was significantly decreased in mice. In hi MC , CE also caused a reduced expression of tryptase. Moreover, in hi MC stimulated by IgE cross‐linking, the release of β‐hexosaminidase was reduced to about 20% by CE . The de novo synthesis of cysteinyl leukotrienes, TNF α, CXCL 8, CCL 2, CCL 3, and CCL 4, was almost completely inhibited by CE . The attenuation of mast cell mediators by CE seems to be related to particular signaling pathways, because we found that activation of the MAP kinases ERK , JNK , and p38 as well as of Akt was strongly reduced by CE . Conclusion CE decreases expression of mast cell–specific mediators in vitro and in vivo and thus is a new plant‐originated candidate for anti‐allergic therapy.