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F cε RI stimulation promotes the differentiation of histamine receptor 1‐expressing inflammatory macrophages
Author(s) -
Novak N.,
Peng W. M.,
Bieber T.,
Akdis C.
Publication year - 2013
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/all.12109
Subject(s) - histamine , flow cytometry , microbiology and biotechnology , receptor , proinflammatory cytokine , histamine h4 receptor , chemistry , stimulation , macrophage , monocyte , immunology , biology , in vitro , inflammation , histamine h2 receptor , endocrinology , biochemistry , antagonist
Background Monocyte differentiation into dendritic cells or macrophages and recruitment to peripheral organs in chronic inflammatory diseases are directed by allergen challenge via F cε RI as well as the nature of soluble factors in the microenvironment. High‐affinity receptor for IgE stimulation of effector cells results in the release of histamine, which acts on various histamine receptors ( HR ) 1‐4, expressed by immune cells. Methods We examined the effect of F cε RI stimulation of human monocytes on H 1 R expression and function of differentiating cells. The m RNA levels of H 1 R , H 2 R and histidine decarboxylase of differentiating cells were detected by quantitative real‐time PCR . Expression of CD 1c, CD 11c, CD 68 and CD 163 was detected by flow cytometry. Amount of histamine, IL ‐6 and IL ‐12p70 in the cell culture was measured with the help of cytometric bead arrays or ELISA assays. Numbers of H 1 R ‐expressing macrophages were evaluated by immunofluorescence double staining of CD 68 and H 1 R on human skin sections. Results We demonstrated that F cε RI stimulation promotes the generation of H 1 R ‐expressing macrophage‐like cells with enhanced histamine biosynthesis and H 1 R ‐mediated proinflammatory properties. Supporting our in vitro findings, high numbers of H 1 R ‐expressing CD 68 pos macrophages were detected in the dermis of atopic dermatitis ( AD ) skin lesions. Conclusion Our observations point to a close histamine‐/ HR ‐mediated activation of dermal macrophages, leading to modified cell differentiation and responsiveness via H 1 R , which might contribute to the aggravation of allergic skin inflammation in AD .