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Distant lymph nodes serve as pools of Th 1 cells induced by neonatal BCG vaccination for the prevention of asthma in mice
Author(s) -
Zhang G.,
Wang P.,
Qiu Z.,
Qin X.,
Lin X.,
Li N.,
Huang H.,
Liu H.,
Hua W.,
Chen Z.,
Zhao H.,
Li W.,
Shen H.
Publication year - 2013
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/all.12099
Subject(s) - ovalbumin , medicine , vaccination , immunology , adoptive cell transfer , lymph , immune system , allergy , inflammation , airway , t cell , pathology , surgery
Background Neonatal Bacillus C almette– G uérin ( BCG ) vaccination induces vigorous T‐helper type 1 ( Th 1) responses and inhibits allergy‐related airway dysfunction, but the exact mechanisms remain unclear. The objective of this study was to address where the Th 1 cells induced by neonatal BCG vaccination are generated and stored, and how they are recruited into the inflamed airway for the prevention of allergen‐induced airway inflammation. Methods We vaccinated neonatal C57 BL /6 mice with BCG in a mouse model of asthma and analyzed the expression and function of Th 1 cells in vivo and in vitro . Results BCG vaccination–induced Th1 cells in the local inguinal lymph nodes ( ILN ) migrated into the lungs upon inhaled ovalbumin ( OVA ) challenge in OVA ‐sensitized mice. These CD 4 + T cells in the ILN exhibited potentials of activation, proliferation and cytokine secretion and expressed high levels of CXCR 3. Adoptive transfer of CD 4 + T cells from BCG ‐treated ILN significantly decreased allergic airway responses. In addition, the protective effect of BCG vaccination against allergic airway inflammation was lost upon the excision of the ILN . Conclusions These data demonstrate that ILN serves as a ‘weapon’ pool of Th 1 cells following BCG vaccination, and these cells are ready for the migration into the inflamed lungs upon the allergen challenge, thereby inhibiting allergen‐induced airway disorder.