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Farm‐derived G ram‐positive bacterium S taphylococcus sciuri W 620 prevents asthma phenotype in HDM ‐ and OVA ‐exposed mice
Author(s) -
Hagner S.,
Harb H.,
Zhao M.,
Stein K.,
Holst O.,
Ege M. J.,
Mayer M.,
Matthes J.,
Bauer J.,
Mutius E.,
Renz H.,
Heine H.,
Pfefferle P. I.,
Garn H.
Publication year - 2013
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/all.12094
Subject(s) - house dust mite , immunology , dendritic cell , ovalbumin , tlr2 , cytokine , immune system , biology , innate immune system , medicine , chemistry , allergen , allergy
Abstract Background Farm‐derived dust samples have been screened for bacteria with potential allergo‐protective properties. Among those was S taphylococcus sciuri W 620 ( S. sciuri W620), which we tested with regard to its protective capacities in murine models of allergic airway inflammation. Methods We employed two protocols of acute airway inflammation in mice administering either ovalbumin ( OVA ) or house dust mite extract ( HDM ) for sensitization. Mechanistic studies on the activation of innate immune responses to S . sciuri W 620 were carried out using human primary monocytic dendritic cells (mo DC ) and co‐culture with autologous T cells. Results The allergo‐protective properties of S. sciuri W 620 were proven in a T H 2‐driven OVA model as well as in a mixed T H 1/T H 2 phenotype HDM model as demonstrated by abrogation of eosinophils and neutrophils in the airways after intranasal treatment. In the HDM model, lymph node cell T H 1/ T H 2 signature cytokines were decreased in parallel. Studies on human moDC revealed an activation of TLR 2 and NOD 2 receptors and initiation of DC maturation following incubation with S. sciuri W 620. Cytokine expression analyses after exposure to S. sciuri W 620 showed a lack of IL ‐12 production in mo DC due to missing transcription of the IL ‐12p35 mRNA . However, such DC selectively supported T H 1 cytokine release by co‐cultured T cells. Conclusion and clinical relevance Our proof‐of‐concept experiments verify the screening system of farm‐derived dust samples as suitable to elucidate new candidates for allergo‐protection. S . sciuri W 620 was shown to possess preventive properties on airway inflammation providing the basis for further mechanistic studies and potential clinical implication.