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Single cell immune profiling in transplantation research
Author(s) -
Higdon Lauren E.,
Schaffert Steven,
Khatri Purvesh,
Maltzman Jonathan S.
Publication year - 2019
Publication title -
american journal of transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.89
H-Index - 188
eISSN - 1600-6143
pISSN - 1600-6135
DOI - 10.1111/ajt.15316
Subject(s) - mass cytometry , computational biology , single cell analysis , gene expression profiling , immune system , population , profiling (computer programming) , immune receptor , biology , bioinformatics , computer science , medicine , cell , immunology , gene , gene expression , genetics , phenotype , operating system , environmental health
Recently developed single‐cell profiling technologies hold promise to provide new insights including analysis of population heterogeneity and linkage of antigen receptors with gene expression. These technologies produce complex data sets that require knowledge of bioinformatics for appropriate analysis. In this minireview, we discuss several single‐cell immune profiling technologies for gene and protein expression, including cytometry by time‐of‐flight, RNA sequencing, and antigen receptor sequencing, as well as key considerations for analysis that apply to each. Because of the critical importance of data analysis for high parameter single cell analysis, we discuss essential factors in analysis of these data, including quality control, quantification, examples of methods for high dimensional analysis, immune repertoire analysis, and preparation of analysis pipelines. We provide examples of, and suggestions for, application of these innovative methods to transplantation research.