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Islet damage during isolation as assessed by mi RNA s and the correlation of mi RNA levels with posttransplantation outcome in islet autotransplantation
Author(s) -
Saravanan Prathab Balaji,
Kanak Mazhar A.,
Chang Charles A.,
Darden Carly,
Yoshimatsu Gumpei,
Lawrence Michael C.,
Naziruddin Bashoo
Publication year - 2018
Publication title -
american journal of transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.89
H-Index - 188
eISSN - 1600-6143
pISSN - 1600-6135
DOI - 10.1111/ajt.14615
Subject(s) - islet , medicine , autotransplantation , transplantation , hemoglobin , endocrine system , endocrinology , urology , andrology , insulin , hormone
High‐quality pancreatic islets are essential for better posttransplantation endocrine function in total pancreatectomy with islet autotransplantation ( TPIAT ), yet stress during the isolation process affects quality and yield. We analyzed islet‐enriched microRNAs (mi RNA s) ‐375 and ‐200c released during isolation to assess damage and correlated the data with posttransplantation endocrine function. The absolute concentration of miR‐375, miR‐200c, and C‐peptide was measured in various islet isolation steps, including digestion, dilution, recombination, purification, and bagging, in 12 cases of TPIAT . Posttransplantation glycemic control was monitored through C‐peptide, hemoglobin A 1c , insulin requirement, and SUITO index. The amount of miR‐375 released was significantly higher during enzymatic digestion followed by the islet bagging ( P < .001). Mir‐200c mirrored these changes, albeit at lower concentrations. In contrast, the C‐peptide amount was significantly higher in the purification and bagging steps ( P < .001). Lower amounts of miR‐375 were associated with a lower 6‐month insulin requirement ( P = .01) and lower hemoglobin A 1c ( P = .04). Measurement of the absolute quantity of mi RNA ‐375 and ‐200c released during islet isolation is a useful tool to assess islet damage. The quantity of released mi RNA is indicative of posttransplantation endocrine function in TPIAT patients.