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Ultrasound Imaging Based on Molecular Targeting for Quantitative Evaluation of Hepatic Ischemia–Reperfusion Injury
Author(s) -
Qiu C.,
Yin T.,
Zhang Y.,
Lian Y.,
You Y.,
Wang K.,
Zheng R.,
Shuai X.
Publication year - 2017
Publication title -
american journal of transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.89
H-Index - 188
eISSN - 1600-6143
pISSN - 1600-6135
DOI - 10.1111/ajt.14345
Subject(s) - medicine , andrographolide , microbubbles , ultrasound , molecular imaging , ischemia , reperfusion injury , magnetic resonance imaging , targeted therapy , nuclear medicine , pharmacology , radiology , in vivo , microbiology and biotechnology , cancer , biology
The aim of the present study was to quantitatively diagnose and monitor the therapy response of hepatic ischemia–reperfusion injury ( IRI ) with the use of targeted ultrasound ( US ) imaging. Targeted microbubbles ( MB s) were fabricated, and the binding of intracellular adhesion molecule 1 (ICAM ‐1) antibodies to MB s was observed. To establish a quantitative method based on targeted US imaging, contrast‐enhanced US was applied for IRI rats. After andrographolide treatment, the IRI rats were subjected to the quantitative targeted US imaging for a therapeutic effect. Effective binding of ICAM ‐1 antibodies to MB s was observed. According to the quantitative targeted US imaging, the ICAM ‐1 normalized intensity difference ( NID ) in the IRI rats (38.74 ± 15.08%) was significantly higher than that in the control rats (10.08 ± 2.52%, p = 0.048). Further, different degrees of IRI (mild IRI , moderate to severe IRI ) were distinguished by the use of the NID (37.14 ± 2.14%, 22.34 ± 1.08%, p = 0.002). Analysis of mRNA expression demonstrated the accuracy of analyzing the NID by using quantitative targeted US imaging (R 2 = 0.7434, p < 0.001). Andrographolide treatment resulted in an obviously weakened NID of ICAM ‐1 (17.7 ± 4.8% vs 34.2 ± 6.6%, p < 0.001). The study showed the potential of the quantitative targeted US imaging method for the diagnosis and therapeutic monitoring of IRI .