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Transcriptomic Signature of the CD 24 hi CD 38 hi Transitional B Cells Associated With an Immunoregulatory Phenotype in Renal Transplant Recipients
Author(s) -
Bigot J.,
Pilon C.,
Matig M.,
Grondin C.,
Leibler C.,
Aissat A.,
Pirenne F.,
Cohen J. L.,
Grimbert P.
Publication year - 2016
Publication title -
american journal of transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.89
H-Index - 188
eISSN - 1600-6143
pISSN - 1600-6135
DOI - 10.1111/ajt.13904
Subject(s) - medicine , transcriptome , signature (topology) , computational biology , genetics , gene , gene expression , biology , geometry , mathematics
The role of B cells after transplant regarding allograft rejection or tolerance has become a topic of major interest. Recently, in renal transplant recipients, a B cell signature characterized by the overexpression of CD 19 + CD 38 hi CD 24 hi transitional B cells has been observed in operationally tolerant patients and in belatacept‐treated patients with significantly lower incidence of donor‐specific antibodies. The phenotypic and functional characterization of these transitional B cells is far from exhaustive. We present the first transcriptomic and phenotypic analysis associated with this cell phenotype. Three populations were studied and compared: (i) transitional CD 24 hi CD 38 hi , (ii) CD 24 + CD 38 − , and (iii) CD 24 int CD 38 int B cells. Transcriptome bioinformatic analysis revealed a particular signature for the CD 24 hi CD 38 hi population. Phenotypic analysis showed that CD 24 hi CD 38 hi transitional B cells also expressed CD 9, CD 10, CD 1b and inducible T cell costimulator ligand ( ICOS ‐L) markers. In addition, we found enrichment of IL ‐10 + cells among CD 24 hi CD 38 hi cells expressing ICOS ‐L and CD 1b, the latter showing regulatory properties. Renal transplant recipients treated with belatacept exhibited significant expression of CD 1b. Our results show that transitional CD 24 hi CD 38 hi B cells exhibit a distinct and specific profile, and this could be helpful for understanding of immune‐regulatory mechanisms and immune monitoring in the field of organ transplant and autoimmune disease.