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Retracted: Characterization of Human CD8 + TCR − Facilitating Cells In Vitro and In Vivo in a NOD/SCID/IL2rγ null Mouse Model
Author(s) -
Huang Y.,
Elliott M. J.,
Yolcu E. S.,
Miller T. O.,
Ratajczak J.,
Bozulic L. D.,
Wen Y.,
Xu H.,
Ratajczak M. Z.,
Ildstad S. T.
Publication year - 2016
Publication title -
american journal of transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.89
H-Index - 188
eISSN - 1600-6143
pISSN - 1600-6135
DOI - 10.1111/ajt.13511
Subject(s) - homing (biology) , cd8 , immunology , progenitor cell , haematopoiesis , bone marrow , transplantation , cxcr4 , cytotoxic t cell , biology , cancer research , stem cell , immune system , medicine , microbiology and biotechnology , in vitro , chemokine , ecology , biochemistry
CD8 + /TCR − facilitating cells (FCs) in mouse bone marrow (BM) significantly enhance engraftment of hematopoietic stem/progenitor cells (HSPCs). Human FC phenotype and mechanism of action remain to be defined. We report, for the first time, the phenotypic characterization of human FCs and correlation of phenotype with function. Approximately half of human FCs are CD8 + /TCR − /CD56 negative (CD56 neg ); the remainder are CD8 + /TCR − /CD56 bright (CD56 bright ). The CD56 neg FC subpopulation significantly promotes homing of HSPCs to BM in nonobese diabetic/severe combined immunodeficiency/IL‐2 receptor γ‐chain knockout mouse recipients and enhances hematopoietic colony formation in vitro . The CD56 neg FC subpopulation promotes rapid reconstitution of donor HSPCs without graft‐versus‐host disease (GVHD); recipients of CD56 bright FCs plus HSPCs exhibit low donor chimerism early after transplantation, but the level of chimerism significantly increases with time. Recipients of HSPCs plus CD56 neg or CD56 bright FCs showed durable donor chimerism at significantly higher levels in BM. The majority of both FC subpopulations express CXCR4. Coculture of CD56 bright FCs with HSPCs upregulates cathelicidin and β‐defensin 2, factors that prime responsiveness of HSPCs to stromal cell–derived factor 1. Both FC subpopulations significantly upregulated mRNA expression of the HSPC growth factors and Flt3 ligand. These results indicate that human FCs exert a direct effect on HSPCs to enhance engraftment. Human FCs offer a potential regulatory cell‐based therapy for enhancement of engraftment and prevention of GVHD.