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Cell‐Free DNA: An Upcoming Biomarker in Transplantation
Author(s) -
Gielis E. M.,
Ledeganck K. J.,
De Winter B. Y.,
Del Favero J.,
Bosmans J.L.,
Claas F. H. J.,
Abramowicz D.,
Eikmans M.
Publication year - 2015
Publication title -
american journal of transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.89
H-Index - 188
eISSN - 1600-6143
pISSN - 1600-6135
DOI - 10.1111/ajt.13387
Subject(s) - medicine , cell free fetal dna , transplantation , biomarker , dna , computational biology , genetics , biology , pregnancy , fetus , prenatal diagnosis
After organ transplantation, donor‐derived cell‐free DNA (ddcfDNA) can be detected in the recipient's blood and urine. Different ddcfDNA quantification techniques have been investigated but a major breakthrough was made with the introduction of digital droplet PCR and massive parallel sequencing creating the opportunity to increase the understanding of ddcfDNA kinetics after transplantation. The observations of increased levels of ddcfDNA during acute rejection and even weeks to months before histologic features of graft rejection point to a possible role of ddcfDNA as an early, noninvasive rejection marker. In this review, we summarize published research on ddcfDNA in the transplantation field thereby elaborating on its clinical utility.