Premium
Comparative Analysis of Assays for Detection of Cell‐Mediated Immunity Toward Cytomegalovirus and M. tuberculosis in Samples From Deceased Organ Donors
Author(s) -
Schmidt T.,
Schub D.,
Wolf M.,
Dirks J.,
Ritter M.,
Leyking S.,
Singh M.,
Zawada A. M.,
BlaesEise A.B.,
Samuel U.,
Sester U.,
Sester M.
Publication year - 2014
Publication title -
american journal of transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.89
H-Index - 188
eISSN - 1600-6143
pISSN - 1600-6135
DOI - 10.1111/ajt.12787
Subject(s) - elispot , medicine , quantiferon , immunology , serology , mycobacterium tuberculosis , virology , tuberculin , tuberculosis , antibody , immune system , t cell , latent tuberculosis , pathology
Cell‐mediated immunity assays could be valuable for risk assessment of organ donors, but no data exist on their feasibility in deceased donors. In this study, 105 deceased donors (52.3 ± 16.9 years) were screened at the time of organ procurement. Pathogen‐specific stimulation was performed using a cytomegalovirus (CMV) lysate, tuberculin (purified protein derivative [PPD]) and soluble Mycobacterium tuberculosis ‐specific ESAT‐6/CFP‐10 proteins in combination with an in‐house fluorescence‐activated cell sorting (FACS) assay or commercial assay formats (QuantiFERON‐CMV/TB for ELISA, T‐SPOT. TB for ELISPOT). CMV‐IgG antibody titers were determined as gold standard for CMV infection; 51.4% of samples were CMV seropositive. Indeterminate results were observed in 47.6% of ELISA, 12.5% of FACS and 0% of ELISPOT assays. Agreement with serology was highest for FACS (95.6%, κ = 0.91), followed by ELISPOT (84.0%, κ = 0.68) and ELISA (80.0%, κ = 0.60). Agreement between ELISA and serology increased if the CMV lysate was used as stimulus (96.7%, κ = 0.92). Among the T cell assays, agreement between ELISPOT and FACS was highest (κ = 0.70). PPD‐positive results among valid samples differed between assays (26.5% for ELISA, 23.1% for FACS and 50.5% for ELISPOT); 2.0% were QuantiFERON‐TB positive, 3.3% were ESAT‐6/CFP‐10‐positive in FACS and 13.4% were positive in the T‐SPOT. TB assay. In conclusion, cellular immunity may be analyzed from samples of deceased donors, although the assays differ in the rate of positivity and indeterminate results.