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Decidual memory T‐cell subsets and memory T‐cell stimulatory cytokines in early‐ and late‐onset preeclampsia
Author(s) -
Kieffer Tom E. C.,
Laskewitz Anne,
Vledder Annegé,
Scherjon Sicco A.,
Faas Marijke M.,
Prins Jelmer R.
Publication year - 2020
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.13293
Subject(s) - preeclampsia , decidua , cd8 , medicine , t cell , endocrinology , immunology , andrology , memory t cell , biology , c c chemokine receptor type 7 , interleukin 10 , immune system , fetus , placenta , pregnancy , chemokine , genetics , chemokine receptor
Abstract Problem Preeclampsia is a major cause of fetal and maternal mortality and morbidity. Disturbed fetal‐maternal immune tolerance, and therewith memory T cells, might be involved in its etiology. This study aims to give insight into memory T‐cell populations and its associated cytokines in the decidual layers in early‐onset preeclampsia (EO‐PE) and late‐onset preeclampsia (LO‐PE). Method of Study Lymphocytes were isolated from the decidua parietalis and basalis from EO‐PE (n = 6), LO‐PE (n = 8) and healthy (n = 15) pregnancies. CD4 + and CD8 + central‐ (CCR7 + ), effector‐ (CCR7 − ), tissue resident‐ (CD103 + ), and regulatory‐ (Foxp3 + ) memory cell (CD45RO + ) populations and their activation status (CD69 + ) were analyzed using flow cytometry. qRT‐PCR analysis was performed on decidua parietalis and basalis biopsies to detect mRNA expression of interferon‐gamma , interleukin‐1B , IL2 , IL6 , IL7 , IL8 , IL10 , IL15 , and IL23 . Results CD4 + central‐memory (CM) cell proportions were lower in the decidua parietalis in LO‐PE ( P < .0001) and EO‐PE ( P < .01) compared to healthy pregnancies. CD8 + memory ( P < .05) and CD8 + CM ( P < .01) cell proportions were also lower in the decidua parietalis in EO‐PE compared to healthy pregnancies. This was accompanied by higher IL15 ( P < .05) and IL23 ( P < .05) and lower IL7 ( P < .05) mRNA expression in decidua basalis biopsies from EO‐PE compared to healthy pregnancies, analyzed by qPCR. Conclusion In conclusion, decidual memory T‐cell proportions, their activation status, and associated cytokines are altered in preeclampsia and might therefore be involved in fetal‐maternal immune tolerance and the pathophysiology of preeclampsia.