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Decidual stromal cells maintain decidual macrophage homeostasis by secreting IL‐24 in early pregnancy
Author(s) -
Yang HuiLi,
Wang ChengJie,
Lai ZhenZhen,
Yang ShaoLiang,
Zheng ZiMeng,
Shi JiaWei,
Li MingQing,
Shao Jun
Publication year - 2020
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.13261
Subject(s) - stromal cell , decidual cells , flow cytometry , gene knockdown , microbiology and biotechnology , decidua , biology , cytokine , thymic stromal lymphopoietin , macrophage , apoptosis , chemistry , immunology , cancer research , in vitro , placenta , fetus , pregnancy , biochemistry , genetics
Problem The state of self‐renewal and self‐maintain of decidual macrophages would be important for immune homeostasis at the maternal‐fetal interface. The roles of interleukin (IL)‐24 derived from decidual stromal cells (DSCs) on decidual macrophages have not been explored. Method of study IL‐24 expression in DSCs was interfered by lentivirus, and the transcription levels of IL‐24 in DSCs were verified by real time (RT)‐PCR. The levels of IL‐24 receptors were determined by flow cytometry assays. The effect of recombination human IL‐24 (rhIL‐24) on the differentiation and apoptosis of macrophages was analyzed by flow cytometry in vitro. The viability of macrophages was detected by Cell Counting Kit‐8 assays. Results The growth of DSCs was not affected obviously only by IL‐24 knockdown while the growth of knockdown DSCs was inhibited significantly after co‐cultured with decidual macrophages. The levels of IL‐24 receptors (IL‐20R1 and IL‐22R1) were moderately to highly expressed on decidual macrophages and human macrophage cell line U937. The differentiation of decidual macrophages treated by rhIL‐24 or co‐cultured with IL‐24 knockdown DSCs was not affected. Both apoptosis and viability of U937 cells were promoted by rhIL‐24. The ratio of Bcl‐2/Bax was down‐regulated and Ki‐67 was up‐regulated by IL‐24 treatment. The expression of Bcl‐2/Bax was up‐regulated while Ki‐67 was down‐regulated in U937 cells after co‐cultured by IL‐24 knockdown DSCs. Conclusion IL‐24 secreted by DSCs promotes the renewal and homeostasis of decidual macrophages possibly via down‐regulating the ratio of Bcl‐2/Bax and up‐regulating of the expression of Ki‐67 in early pregnancy.