Premium
Distribution of dendritic cells in the septate uterus: An immunological perspective
Author(s) -
Negishi Yasuyuki,
Kato Masahiko,
Ono Shuichi,
Kuwabara Yoshimitsu,
Morita Rimpei,
Takahashi Hidemi,
Takeshita Toshiyuki
Publication year - 2020
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.13241
Subject(s) - chemokine , myometrium , uterus , andrology , stromal cell , endometrium , immunohistochemistry , biology , immune system , adenomyosis , hysteroscopy , medicine , immunology , gynecology , pathology , endocrinology
Problem Septate uterus is associated with spontaneous abortion. Surgical intervention of the uterine septa (US) is frequently performed following spontaneous abortion; however, immunological mechanisms for spontaneous abortion in patients with septate uterus remain completely unknown. Method of study A total of 12 women with septate uterus who underwent hysteroscopic metroplasty and 10 women with uterine leiomyoma who underwent total hysterectomy were enrolled as the experimental and control groups, respectively. Immune cells, dendritic cells (DCs), macrophages, T cells, natural killer cells, invariant natural killer cells, and chemokine receptors in US and uterine myometrium tissue (UMT) were analyzed using flow cytometry and immunohistochemical staining. Additionally, the chemokine production of macrophage inflammatory protein 1 alpha (MIP‐1α), regulated upon activation normal T‐cell express sequence (RANTES), and macrophage inflammatory protein 3 beta (MIP‐3β) from the viable cells obtained from the US and UMT samples was evaluated in an ex vivo study. Results The percentage of CD141 + DCs in US was significantly lower than that in UMT. Both US and UMT showed CCR1 and CCR5 expression on CD141 + DCs; however, the production of chemokines, MIP‐1α, RANTES, and MIP‐3β was abundant in UMT‐obtained viable cells. Conclusion The accumulation of CD141 + DCs was lower in US than that in UMT. This phenomenon may be caused by low chemokine productions in US. Our findings support the benefit of surgical intervention for septate uterus—that is, the elimination of inappropriate implantation sites.