Premium
Pregnancy favors circulating IL‐21–secreting T FH ‐like cell recovery in ARV‐treated HIV‐1–infected women
Author(s) -
Kasahara Taissa M.,
Monteiro Clarice,
Hygino Joana,
Cafasso Marcos O. S. D.,
Oyamada Hugo A. A.,
Andrade Regis M.,
Ferreira Orlando,
Leite Simone,
Silva Vander G.,
Gupta Sudhir,
Bento Cleonice A. M.
Publication year - 2020
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.13204
Subject(s) - immunology , medicine , toxoid , peripheral blood mononuclear cell , antibody , hbsag , immune system , pregnancy , tetanus , cd38 , t cell , immunization , biology , virus , vaccination , in vitro , hepatitis b virus , genetics , biochemistry , stem cell , cd34
Problem Pregnancy appears to favor maternal antibody production. In contrast, by damaging follicular helper T cells (T FH ), HIV‐1 infection compromises protective humoural immune response. Therefore, we aimed to investigate the frequency of different T FH ‐like cells in HIV‐infected pregnant women (PW) before and after antiretroviral (ARV) therapy. Method of study Peripheral blood mononuclear cells, CD4 + T and B cells, were obtained from asymptomatic HIV‐1–infected non‐PW and PW just before and after ARV therapy. In some experiments, healthy HIV‐1–negative PW were also tested. The frequency of different T FH ‐like cell subsets was determined by flow cytometry. The plasma titers of IgG anti‐tetanus toxoid (TT), anti‐HBsAg, and anti‐gp41 were determined by ELISA. The in vitro production of total IgG, IL‐21, and hormones (estrogen and progesterone) was quantified also by ELISA. Results Our results demonstrate that antiretroviral (ARV) therapy was more efficient in elevating the percentage of circulating IL‐21–secreting T FH cells in HIV‐1–infected pregnant women (PW) than in non‐pregnant patients (nPW). Moreover, in co‐culture systems, CD4 + T cells from ART‐treated PW were more efficient in assisting B cells to produce IgG production. The in vivo anti‐HBsAg IgG titers after ARV therapy were also significantly higher in PW, and their levels were directly associated with both IL‐21 + T FH frequency and plasma concentration of estrogen. Conclusion In summary, our results suggest that pregnancy favors the recovery of T FH ‐like cells after ARV therapy in HIV‐1–infected women, which could help these mothers to protect their newborns from infectious diseases by transferring IgG across the placenta.