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In vitro antioxidation effect of Quercetin on sperm function from the infertile patients with leukocytospermia
Author(s) -
Diao Ruiying,
Gan Huimei,
Tian Fuying,
Cai Xueyong,
Zhen Wanhua,
Song Xiaolu,
Duan YongGang
Publication year - 2019
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.13155
Subject(s) - sperm , quercetin , andrology , semen , sperm motility , acrosome reaction , motility , semen analysis , biology , acrosome , antioxidant , infertility , medicine , biochemistry , genetics , pregnancy
Problem Quercetin has been shown to display intensive antioxidant activity against ROS‐mediated damage in chilled semen, and the effects and molecular mechanisms of Quercetin on sperm function in the infertile patients with leukocytospermia remain largely unknown. Methods Semen samples were collected from the infertile men with leukocytospermia (n = 56) and fertile men (n = 44). Computer‐assisted semen analysis (CASA) was used to determine sperm motility before and after Quercetin incubation (10 μmol/L). Changes in H 2 O 2 , sperm mitochondrial DNA (mtDNA), cytochrome B (Cty B), and NADH dehydrogenase 5 (NADH5) contents were measured. Furthermore, hyperactivated motility (HA) and acrosome reaction rates were detected after the stimulation by progesterone with or without Quercetin, respectively. Results Quercetin could significantly improve sperm motility from the leukocytospermic patients. The level of H 2 O 2 was significantly decreased in the supernatant of leukocytospermic patients after Quercetin treatment. The content of mtDNA in sperm was significantly decreased, whereas the contents of Cyt B and NADH 5 in sperm were significantly increased. Sperm hyperactivated motility and acrosome reaction induced by progesterone were enhanced by Quercetin in sperm from the infertile men with leukocytospermia. Conclusion These data indicate Quercetin could display protective effects against oxidative damage on sperm from the infertile men with leukocytospermia.